CODE EXAMPLE FOR R

rstudio

rna_fc <- rna %>% select(gene, time,
                         gene_biotype, expression_log) %>%
  group_by(gene, time, gene_biotype) %>%
  summarize(mean_exp = mean(expression_log)) %>%
  pivot_wider(names_from = time,
              values_from = mean_exp) %>%
  mutate(time_8_vs_0 = `8` - `0`, time_4_vs_0 = `4` - `0`)