Python

df[['A','B']] = df[['A','B']].apply(pd.to_datetime) #if conversion required
df['C'] = (df['B'] - df['A']).dt.days

t1 = pd.to_datetime('1/1/2015 01:00')
t2 = pd.to_datetime('1/1/2015 03:30')

print pd.Timedelta(t2 - t1).seconds / 3600.0

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